About SOLARBIO pUC18 Plasmid

OVERVIEW:

SOLARBIO pUC18 Plasmid is a high-quality cloning vector widely used in molecular biology research. It is a plasmid vector designed for the cloning and propagation of DNA fragments in E. coli cells. pUC18 is one of the most popular plasmid vectors due to its ability to facilitate high-efficiency cloning, screening, and expression of recombinant DNA. The plasmid features a high copy number, making it ideal for producing large quantities of plasmid DNA for further applications such as sequencing, protein expression, or gene synthesis. SOLARBIO pUC18 Plasmid is a reliable and versatile tool for researchers working in molecular cloning, gene expression, and other genetic manipulation techniques.

KEY FEATURES:

High Copy Number: pUC18 plasmid is designed with a high copy number, ensuring efficient replication in E. coli cells and the production of large amounts of plasmid DNA for various applications.

Multiple Cloning Sites (MCS): The plasmid contains multiple cloning sites, allowing for easy insertion of foreign DNA fragments into the vector. The MCS is compatible with a wide range of restriction enzymes, providing flexibility in cloning experiments.

Selectable Marker: SOLARBIO pUC18 includes an antibiotic resistance gene, typically for ampicillin resistance, allowing for the selection of successfully transformed E. coli cells and ensuring the propagation of recombinant plasmids.

Small Size and Ease of Transformation: The small size of the pUC18 plasmid, combined with its high transformation efficiency in E. coli, makes it an ideal vector for cloning experiments that require efficient DNA uptake and high expression levels.

Reliable and Versatile: pUC18 is a well-characterized and reliable plasmid vector used for a wide variety of molecular biology applications, including cloning, gene expression, and DNA sequencing.

APPLICATIONS:

Molecular Cloning: SOLARBIO pUC18 Plasmid is widely used in cloning experiments to insert foreign DNA fragments into E. coli cells. The high copy number and multiple cloning sites make it ideal for producing recombinant plasmids.

Gene Expression: The pUC18 plasmid can be used to express recombinant proteins in E. coli cells, making it valuable for protein production in research and industrial applications.

DNA Sequencing: This plasmid is used in sequencing projects, allowing for the amplification and purification of plasmid DNA that can be used for sequencing analysis.

Gene Synthesis and Manipulation: pUC18 is ideal for gene synthesis and various genetic manipulation techniques, allowing researchers to clone synthetic genes and manipulate genetic material for functional studies.

PACKAGING:

SOLARBIO pUC18 Plasmid is available in various quantities, including small-scale (microgram) and larger-scale preparations, making it suitable for both individual research projects and larger-scale experiments.

INSTRUCTIONS FOR USE:

Preparation: To use the pUC18 plasmid, first prepare competent E. coli cells, typically E. coli DH5α or other strains, for transformation.

Cloning: Insert your DNA fragment of interest into the multiple cloning sites of the plasmid using appropriate restriction enzymes. Transform the recombinant plasmid into competent E. coli cells.

Selection: Select transformed cells by growing them on agar plates containing the appropriate antibiotic (e.g., ampicillin). Colonies that contain the recombinant plasmid will grow, while others will not.

Plasmid Extraction: Isolate plasmid DNA from bacterial cultures using a plasmid extraction kit or standard alkaline lysis method, and use it for downstream applications such as sequencing or expression.

Storage: Store SOLARBIO pUC18 Plasmid at -20°C for long-term storage to maintain plasmid integrity.

SAFETY AND HANDLING:

Handle SOLARBIO pUC18 Plasmid with care, following standard molecular biology safety protocols. Wear appropriate personal protective equipment, such as gloves and safety glasses, when handling plasmids to avoid contamination and accidental exposure.

CONCLUSION:

SOLARBIO pUC18 Plasmid is an essential tool for molecular biology research, providing a reliable and efficient method for cloning, gene expression, and genetic manipulation. Its high copy number, multiple cloning sites, and selectable marker make it ideal for a wide range of applications, from protein production to DNA sequencing. Whether used for molecular cloning or gene synthesis, SOLARBIO pUC18 Plasmid ensures consistent and reproducible results, making it an indispensable tool in the lab for genetic research and biotechnology applications.